PROCESS FOR THE PURIFICATION OF RECOMBINANT HUMAN ERYTHROPOIETIN (EPO), EPO THUS PURIFIED AND PHARMACEUTICAL COMPOSITIONS COMPRISING SAME

摘要

Disclosed is a method of purifying glycosylated erythropoietin (EPO) isoforms from a complex protein mixture, wherein the method comprises an anion exchange chromatography step and a cation exchange chromatography step, which are separated by a reverse phase (RP) chromatography step.