| 摘要 |
The invention relates to a method for identifying the bacteria Oenococcus, Leuconostoc, Pediococcus and Lactobacillus in a fermentation sample, comprising the steps of: a) obtaining a sample from a fermentation; b) eliminating colorants, phenols and other substances inhibiting the PCR of the sample; c) lysis of the bacteria present in the sample; d) extraction of the DNA from the samples; e) PCR amplification of the extracted DNA using universal dividers designed for an objective region of the gene 16S rRNA; f) digestion of the amplified DNA using a restriction solution with enzymes selected by theoretic analysis; g) incubation; and h) electrophoresis of the obtained fragments. The invention also relates to a kit for implementing the described method. |
