ASSAY FOR MODULATOR OF PARKIN ACTIVITY USING NEW SUBSTRATE

摘要

PROBLEM TO BE SOLVED: To provide an in vitro method for assessing specificity of a positive modulator of parkin activity.SOLUTION: The invention provides in vitro and cell-based assays for parkin activity, in which parkin-mediated ubiquitination of the S5a subunit of the 26S proteasome is measured, or ubiquitination of troponin 1. The assays may be used to screen for agents that modulate parkin protein ligase activity.