METHOD FOR PRODUCING PLASMIN BY LIQUID FERMENTATION OF CORDYCEPS MILITARIS

摘要

Provided is a method for producing plasmin by liquid fermentation of Cordyceps militaris, comprising the steps of: (1) inoculating strains of Cordyceps militaris into the PD liquid fermentation medium, activating the culturing of the strains, then transferring them to a seed fermentation medium and obtaining a seed solution from seed culture; (2) inoculating the seed solution into a liquid fermentation medium at a volume ratio of 5-15% and culturing for 20 - 30 h, then adding an expansin solution and further culturing for 120 - 168 h, and obtaining fermentation supernatant of plasmin by separating and removing Cordyceps militaris mycelium; (3) obtaining plasmin of Cordyceps militaris by extracting extracellular plasmin of Cordyceps militaris from the fermentation supernatant of plasmin. A plant expansin and an optimized fermentation method are used in the liquid fermentation production of plasmin of Cordyceps militaris in the invention, which increases the liquid fermentation yield of plasmin of Cordyceps militaris significantly, by more than 2.69 times compared with traditional fermentation production methods with a yield of 93280U per liter of fermentation broth; there is an excellent industrial application prospect.