| 摘要 |
PURPOSE: A method for screening a material which prevents gray hair using a transgenic cell line and a vitiligo model mouse(C57bl/6-Mitfmi-vit) and a composition containing the same are provided to simultaneously recognize MITF, tyrosinase, and TRP2 changes in gene, protein, and melanin levels. CONSTITUTION: A melan-a melanocyte cell line tyrosinase-GLuc(KCLRF-BP-00163) is prepared by transforming with an expression vector, pTyrosinase-GLuc. A method for screening a material which prevents gray hair comprises: a step of treating a candidate material which prevents gray hair to the transgenic melanocyte cell line; a step of measuring the production quantity of GLuc existing in a medium; a step of applying the candidate material onto the back of a vitiligo mouse; and a step of measuring the melanin quantity of newly grown hair from the back of vitiligo mouse. |
