发明名称 |
METHOD FOR EXTRACTING AND ANALYSING OF INTRACELLULAR METABHOLIC PROFILE IN MICROORGANISMS |
摘要 |
PURPOSE: An extraction and analysis method of microorganisms for acquiring an intracellular metabolic profile is provided to obtain a metabolic profile of high productivity and high yield and to construct an optimal method for preparing intracellular metabolites. CONSTITUTION: A method for acquiring an intracellular metabolic profile in microorganisms comprises the steps of: suspending microorganism biomass in cold methanol and freezing the biomass; thawing the biomass and repeating the steps; collecting a suspension of the metabolites released from the biomass; and performing mass spectrometry of the suspension and identifying the intracellular metabolites. |
申请公布号 |
KR20130115815(A) |
申请公布日期 |
2013.10.22 |
申请号 |
KR20120038484 |
申请日期 |
2012.04.13 |
申请人 |
SOGANG UNIVERSITY RESEARCH FOUNDATION |
发明人 |
LEE, JIN WON;PARK, CHANG HUN;LU MING SHOU;LEE, SOO JIN;KIM, BO RIM |
分类号 |
C12Q1/02;C12N1/20;C12P1/04 |
主分类号 |
C12Q1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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