SUPERPARAMAGNETIC NANOPARTICLE AND ADENO-ASSOCIATED VIRUS COMPOSITE, AND GENE DELIVERY TO CELL USING THE SAME

摘要

PURPOSE: A composite of superparamagnetic nanoparticles and adeno-associated virus (AAV) is provided to effectively and quickly infect a target cell, to induce physiological reaction through the expression of a transgene, and to enable various gene therapies. CONSTITUTION: A composite contains superparamagnetic nanoparticles coated with heparin and an AAV vector conjugated to the surface of the nanoparticles. A method for improving gene delivery efficiency to cells comprises the step of culturing a gene delivery system and cells under a magnetic field. A method for controlling gene delivery rate to cells comprises the steps of: culturing the gene delivery system and the cells under the magnetic field and controlling exposure time of the magnetic field. [Reference numerals] (AA) Heparin; (BB) AAV2 or AAVr3.45