发明名称 |
Rapid generation of antibodies |
摘要 |
High efficient methods for producing an antibody molecule that binds an antigen are described. The methods include obtaining a population of PBMC enriched for CD19highCD3negCD20low to negCD38highCD27high cells from a mammal exposed to an antigen from sample of cells enriched for PBMC. The cells are isolated from a sample obtained at a time that the fraction of PBMC expressing antibody reactive to the antigen is at a high level. Sequences encoding heavy and light chain variable domains are prepared in a manner that allow production of molecules with natural heavy and light chain pairing.
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申请公布号 |
US8563305(B2) |
申请公布日期 |
2013.10.22 |
申请号 |
US20090433832 |
申请日期 |
2009.04.30 |
申请人 |
AHMED RAFI;MILLER JOSEPH;WILSON PATRICK C.;CAPRA J. DONALD;WRAMMERT JENS;OKLAHOMA MEDICAL RESEARCH FOUNDATION;EMORY UNIVERSITY |
发明人 |
AHMED RAFI;MILLER JOSEPH;WILSON PATRICK C.;CAPRA J. DONALD;WRAMMERT JENS |
分类号 |
C12N5/02 |
主分类号 |
C12N5/02 |
代理机构 |
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地址 |
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