| 摘要 |
PROBLEM TO BE SOLVED: To provide fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems.SOLUTION: In some embodiments, a fluidic connector having a fluid path to connect two independent channels formed in a substrate is used so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods (e.g., 1 year) prior to use. Prior to connection of the fluid connector and the substrate, the fluid path may be filled with a sample (e.g., blood). The sample may be obtained, for example, by pricking a finger of a user until blood is drawn from the finger into the fluid path (e.g., by capillary force). |
