METHOD FOR PREPARING A NUCLEIC ACID WITH HIGH PRECISION USED IN DETECTING A NUCLEIC ACID WITH A NUCLEIC ACID POLYMERASE

摘要

The present invention relates to a method for preparing a nucleic acid with high precision, which is advantageous in that a nucleic acid polymerase is used to add a terminator to the nucleic acids to be used for analysis prior to the nucleic acid polymerization reaction such as PCR reaction or real time quantitative PCR reaction for detecting an infinitesimal nucleic acid, so that non-singular priming occurring competitively with amplification reaction of the target nucleic acid is basically eliminated, thus precisely detecting the infinitesimal target nucleic acid and precisely measuring the concentration of the target nucleic acid.