METHOD FOR QUANTIFYING SUBFRACTION OF CHOLESTEROL (-C) IN HIGH-DENSITY LIPOPROTEIN (HDL)

摘要

Provided is a method for quantifying cholesterol in the whole HDL-C and HDL subfractions, i.e., ApoE-containing HDL-C and ApoE-deficient HDL-C, separately or simultaneously. A method for enzymatically quantifying cholesterol in an ApoE-deficient HDL in a selective manner by adding a surfactant comprising a polyoxyethylene benzyl phenyl ether derivative to a sample to be tested at a final concentration of 0.05 to 0.10%, allowing cholesterol esterase and cholesterol oxidase to act on the sample, and then quantifying generated hydrogen peroxide; and a method for enzymatically quantifying cholesterol in an ApoE-containing HDL in a selective manner by adding a surfactant comprising a polyoxyethylene benzyl phenyl ether derivative to a sample to be tested at a final concentration of 0.15 to 0.75%, allowing cholesterol esterase and cholesterol oxidase to act on the sample, and then quantifying generated hydrogen peroxide.