METHOD FOR PRODUCING A TOXIC PROTEIN BY USING LACI PROTEIN MUTANT

摘要

The present invention relates to a method for producing a toxic protein by using LacI protein mutant, a gene coding LacI protein mutant, a recombination vector including the gene, a host cell and the host cell transformed into the recombination vector, which controls precisely the expression of LacI mutant gene depending on the concentration of Rhamnose by using Rha promoter controlled by Rhamnose, so as to optimize the expression of the target protein to produce in a coliform bacillus cell, thus increasing productivity, and having great usefulness for the expression of a recombination toxic protein in a host cell including coliform bacillus.