RECOMBINANT C1 B-GLUCOSIDASE FOR PRODUCTION OF SUGARS FROM CELLULOSIC BIOMASS

摘要

The invention relates to expression of a recombinant C1 &bgr;-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting cellobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 &bgr;-glucosidase, operably linked to heterologous promoter, under conditions in which &bgr;-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said &bgr;-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.