OPTIMIZED MEDIUM AND FERMENTATION CONDITIONS FOR XYLANASE MASS PRODUCTION BY RECOMBINANT PICHIA PASTORIS,申请号KR20120035213-传众专利搜索

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发明名称	OPTIMIZED MEDIUM AND FERMENTATION CONDITIONS FOR XYLANASE MASS PRODUCTION BY RECOMBINANT PICHIA PASTORIS
摘要	PURPOSE: Xylanase produced by recombinant Pichia pastoris is provided to largely reduce the amount of chemicals needed for bleaching craft pulp, thereby being used as an eco-friendly bleaching agent. CONSTITUTION: A medium composition for recombinant Pichia pastoris strains for producing xylanase contains 7.56-15.22 g/L of wheat bran, 0.13-1.46 g/L of potassium dihydrogen phosphate (KH_2PO_4), and 0.02-0.25 g/L of potassium hydrogen sulfate (KHSO_4). A method for producing xylanase comprises the step of: putting the recombinant Pichia pastoris strains in the medium composition; and culturing the recombinant Pichia pastoris strains. The recombinant Pichia pastoris strains are cultured at 24-27°C, a pH concentration of 5.5-6.5, a stirring rate of 160-180 rpm, and an air flow rate of 0.5-1.5 vvm under the presence of 0.5-1.25% methanol.
申请公布号	KR20130113006(A)	申请公布日期	2013.10.15
申请号	KR20120035213	申请日期	2012.04.05
申请人	INDUSTRIAL COOPERATION FOUNDATION CHONBUK NATIONAL UNIVERSITY	发明人	JEONG, YONG SEOB;MINYUAN ZHANG;PARK, SEUNG MOON
分类号	C12N1/16;C12N1/38;C12N9/24;C12P21/00	主分类号	C12N1/16
代理机构		代理人
主权项
地址

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