RECOMBINANT PROTEINS, POLYNUCLEOTIDES AND VACCINES TO COMBAT BOVINE HERPESVIRUS

摘要

The present technology describes the production of a vaccine containing recombinant proteins comprising 4 epitopes of the main antigenic proteins of each of two bovine herpesviruses, namely BoHV-1 (Bovine herpesvirus 1) and BoHV-5 (Bovine herpesvirus 5), produced in a prokaryotic system and/or a eukaryotic system using recombinant virus generated on the MVA (Modified Vaccinia Ankara) platform. Specifically, each recombinant protein contains 4 epitopes produced linearly, one with the BoHV-1 epitopes and the other with the BoHV-5 epitopes, and the epitopes linked with the use of flexible glycine and serine rings to connect the desired regions and to allow suitable tertiary conformation of each fragment, resulting in two multi-epitope proteins with potential to immunize ruminants (especially bovines) against the BoHV-1 and BoHV-5 viruses, for controlling infection and viral dissemination and reducing the level of financial loss caused by two bovine diseases of great significance for the worldwide livestock sector, namely bovine infectious rhinotracheitis and bovine encephalitis.