发明名称 |
RECOMBINANT PLASMID pAd-SM, CODING HUMAN PROTEINS SOX2 AND C-MYC, BEING BASE FOR PRODUCTION OF VIRULENT ADENOVIRUSES DESIGNED FOR PRODUCTION OF INDUCED PLURIPOTENT HUMAN CELLS |
摘要 |
FIELD: biotechnologies.SUBSTANCE: genetic structure pAd-SM is produced, being built by homological recombination of the vector pAdEasy-1, containing the main part of genome of adenovirus and plasmid pAdTrack-CMV, where cDNA fragments of human genes SOX2 and C-MYC are placed, being connected with a nucleotide sequence that codes P2A-peptide. The plasmid pAd-SM produced as a result of homological recombination contains a fragment SOX2-P2A-C-MYC under control of a constitutive promotor CMV.EFFECT: possibility to produce adenovirus particles for simultaneois delivery of genes and expression of proteins SOX2 and C-MYC in human cells, pAd-SM contains a gene that codes a fluorescent protein EGFP, under control of a CMV promotor, which makes it possible to track transduction of cells and elimination of virus DNA from a cell.3 cl, 1 dwg |
申请公布号 |
RU2495127(C2) |
申请公布日期 |
2013.10.10 |
申请号 |
RU20120101366 |
申请日期 |
2012.01.13 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE "NOVOSIBIRSKIJ NAUCHNO-ISSLEDOVATEL'SKIJ INSTITUT PATOLOGII KROVOOBRASHCHENIJA IMENI AKADEMIKA E.N. MESHALKINA" MINISTERSTVA ZDRAVOOKHRANENIJA ROSSIJSKOJ FEDERATSII (FGBU "NNIIPK IM. AKAD. E.N. MESHALKINA" MINZDRAVA ROSSII);UCHREZHDENIE ROSSIJSKOJ AKADEMII NAUK INSTITUT TSITOLOGII I GENETIKI SIBIRSKOGO OTDELENIJA RAN;UCHREZHDENIE ROSSIJSKOJ AKADEMII NAUK INSTITUT KHIMICHESKOJ BIOLOGII I FUNDAMENTAL'NOJ MEDITSINY SIBIRSKOGO OTDELENIJA RAN |
发明人 |
MEDVEDEV SERGEJ PETROVICH;SHEVCHENKO ALEKSANDR IGOREVICH;POKUSHALOV EVGENIJ ANATOL'EVICH;ZAKIJAN SUREN MINASOVICH |
分类号 |
C12N15/09;A61K48/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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