SYSTEM AND METHOD FOR CORRECTING PRIMER EXTENSION ERRORS IN NUCLEIC ACID SEQUENCE DATA

摘要

PROBLEM TO BE SOLVED: To provide a method and system for correcting primer extension errors in nucleic acid sequence data.SOLUTION: An embodiment of a method for correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copies of a template molecule is described that comprises: (a) detecting a signal generated in response to incorporation of one or more nucleotides in a sequencing reaction; (b) generating a value for the signal; and (c) correcting the value for the phasic synchrony error using a first parameter and a second carry forward parameter.