| 发明名称 |
RECOMBINANT PLASMID pSN-ZsGreen, CODING SOX2 AND NANOG HUMAN PROTEINS AND FLUORESCENT PROTEIN ZsGreen, DESIGNED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS |
| 摘要 |
FIELD: biotechnologies.SUBSTANCE: plasmid genetic structure pSN-ZsGreen is produced, being built on the basis of a plasmid vector pIRES (Clontech), where fragments of cDNA of human genes SOX2 and NANOG are placed, being connected with a nucleotide sequence coding P2A-peptide and gene cDNA, coding fluorescent protein ZsGreen. Transcription of a single mRNA SOX2-P2A-NANOG-IRES-ZsGreen is carried out from constitutive promotor p CMV IE, providing for high level of mRNA lifelength.EFFECT: availability of P2A and IRES sequences makes it possible to simultaneously translate proteins SOX2, NANOG and ZsGreen from one mRNA molecule.3 cl, 1 dwg, 1 tbl |
| 申请公布号 |
RU2495124(C2) |
申请公布日期 |
2013.10.10 |
| 申请号 |
RU20110149841 |
申请日期 |
2011.12.07 |
| 申请人 |
FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE "NOVOSIBIRSKIJ NAUCHNO-ISSLEDOVATEL'SKIJ INSTITUT PATOLOGII KROVOOBRASHCHENIJA IMENI AKADEMIKA E.N. MESHALKINA" MINISTERSTVA ZDRAVOOKHRANENIJA ROSSIJSKOJ FEDERATSII (FGBU "NNIIPK IM. AKAD. E.N. MESHALKINA" MINZDRAVA ROSSII);UCHREZHDENIE ROSSIJSKOJ AKADEMII NAUK INSTITUT TSITOLOGII I GENETIKI SIBIRSKOGO OTDELENIJA RAN;UCHREZHDENIE ROSSIJSKOJ AKADEMII NAUK INSTITUT KHIMICHESKOJ BIOLOGII I FUNDAMENTAL'NOJ MEDITSINY SIBIRSKOGO OTDELENIJA RAN |
发明人 |
MEDVEDEV SERGEJ PETROVICH;SHEVCHENKO ALEKSANDR IGOREVICH;POKUSHALOV EVGENIJ ANATOL'EVICH;ZAKIJAN SUREN MINASOVICH |
| 分类号 |
C12N15/00;A61K48/00 |
主分类号 |
C12N15/00 |
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