摘要 |
PROBLEM TO BE SOLVED: To provide a rapid, reliable and simple method for detection or species identification of an acid-fast bacterium which is important on clinical diagnosis.SOLUTION: A melting curve analysis is carried out by using a probe whose target nucleic acid is a region having a high bacteria species specificity in dnaJ1 gene in an acid-fast bacterium, whereby Mycobacterium kansasii is specifically detected and identified. |