Method for aerobic production of alanine or a compound arising using alanine

摘要

Producing alanine or a compound produced using alanine, comprises (a) providing a cell, which expresses a recombinant alanine dehydrogenase or its variant, (b) cultivating the cell under aerobic conditions in the presence of an inorganic nitrogen source in an aqueous phase, and (c) contacting the cell with an organic phase, where the cell is a prokaryotic or a lower eukaryotic cell. Independent claims are included for: (1) use of a cell expressing the heterologous alanine-dehydrogenase or its variant, for the production of alanine or a compound produced using alanine under aerobic conditions in the presence of the organic phase; and (2) use of an organic phase for increasing the yield of alanine or the compound formed using alanine comprising contacting prokaryotic or lower eukaryotic cell produced with the alanine or the compound produced using alanine with the organic phase, where the organic phase is a hydrophobic fatty acid ester and a hydrophobic fatty acid, preferably lauric acid methyl ester and oleic acid.