E. COLI TRANSFORMANT, PRODUCTION METHOD FOR FLAVIN-BOUND GLUCOSE DEHYDROGENASE USING SAME, AND MUTANT FLAVIN-BOUND GLUCOSE DEHYDROGENASE

摘要

A flavin-bound glucose dehydrogenase (FAD-GDH) with high substrate specificity for D-glucose is efficiently produced. A gene encoding a mutant FAD-GDH with its N-terminal region, containing an amino acid sequence corresponding to MKITAAIITVATAFASFASA that exists in the N-terminal region, deleted from the amino acid sequence of a wild-type FAD-GDH derived from Mucor is introduced into E. coli to obtain an E. coli transformant. Subsequently, this E. coli transformant is cultured to obtain an FAD-GDH with a specific N-terminal region deleted. The transformant allows the production of a large amount of GDH in a short time as compared with the original microorganism. An FAD-GDH that is less susceptible to the effects of dissolved oxygen and allows accurate measurement of glucose even in the presence of sugar compounds other than glucose in a sample can be efficiently produced.