发明名称 |
Expression vector for establishing highly productive cell and the highly productive cell |
摘要 |
The present invention provides an expression vector which is effective in an efficient establishment of transformed cells which express the aimed protein gene in a high level. An expression vector which has a cassette for expressing the drug selective marker gene containing mRNA destabilizing sequence, at least one element for stabilizing the gene expression and a cassette for expressing the gene of the aimed protein. Preferably, the mRNA destabilizing sequence is derived from AT-rich sequence existing in the 3'-untranslated region of cytokine, interleukin or proto-oncogene, and the element for stabilizing the gene expression is derived from Chinese hamster genome.
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申请公布号 |
US8552170(B2) |
申请公布日期 |
2013.10.08 |
申请号 |
US201013122119 |
申请日期 |
2010.06.04 |
申请人 |
YAMAZAKI TOMOMI;MASUDA KENJI;NISHII SHIGEAKI;KAWAKAMI BUNSEI;TOYO BOSEKI KABUSHIKI KAISHA |
发明人 |
YAMAZAKI TOMOMI;MASUDA KENJI;NISHII SHIGEAKI;KAWAKAMI BUNSEI |
分类号 |
C07H21/04;C12N15/85;C12P21/00 |
主分类号 |
C07H21/04 |
代理机构 |
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