| 摘要 |
Abstract The present disclosure provides compositions and methods for direct transfection of organelle DNA in living cells. More particularly, the present disclosure is based on the use of viral vectors that contain localization signals specific for the target organelle. In one embodiment, a viral vector that has been modified to express on its surface a protein transduction domain and an organelle localization signal is provided. A viral vector comprising a desired recombinant DNA construct is introduced into cell culture or injected into an organism, wherein the viral vector transduces across the cellular membrane through its protein transduction domain and localizes to the cellular organelle by way of the organelle localization/targeting signal introducing the recombinant DNA into the interior of the organelle. WO 2005/001062 PCT/US20041020454 Figure 1A Protein Transduction Domain Targeting Signal Red Fluorescent Protein WO 2005/001062 PCT/US2004/020454 UDiMoorrl Targdrn SiWA1 Red Fbunscal mkLGu ProIit Lumbda, Hbdatid prmtaM lhwitm Doam PTD-MR-gpD 1185 bp Figure l B WO 2005/001062 PCT/US2004/020454 TMIRD ORF Stop aspA Termination Mitochondrial Targeting Signal Trx reverse priming site Protein Transduction Domain gpD Lambda Head Protei Red Fluorescent Protein AmpIlillin Resistance Trc Promoter Region pEXP-TMRD lacO 444 -10 region -35 region pUC Origin of Replication iacl Figure 1C |
