Method of Surface Modification for the Purpose of Enrichment of Phosphorylated Peptides for Analysis by Desorption/Ionization Mass Spectrometry Techniques

摘要

The invention has been based on original way of surface modification of solid substrates for efficient phosphorylated peptide preconcentration from complex peptide mixtures prior to detection based on desorption/ionization mass spectrometry. The subject of protection is the method of surface functionalization by ambient ion landing. The approach was based on electronebulization (electrospray) of solutions of organometallic compounds related to elements of 4B class of periodic table of elements, especially to zirconium, hafnium and titanium. The generated charged electrospray was dried on the fly by passing the heated compartment and focused to a surface forming a stable oxide layer there. The surface modified this way has been used for phosphopeptide enrichment from peptide mixtures prior to desorption/ionization mass spectrometry.