CALCIUM SENSORS AND METHODS FOR DETECTING INTRACELLULAR FREE CALCIUM

摘要

The invention relates to a calcium sensor based on a fusion protein which includes: (i) a first polypeptide domain which is capable of bonding Ca 2+, (ii) a second polypeptide domain of sequence SEQ ID NO. 1 (GFPuv) or a variant thereof that maintains two maximums in the excitation spectrum thereof and at least one maximum in the emission spectrum thereof, and (iii) a flexible peptide that bonds the first domain and the second domain in a covalent manner. The invention also provides a genetically modified non-human animal that includes the fusion protein. Likewise, the invention provides methods for detecting the concentration of intracellular calcium Ca 2+.