发明名称 |
Prokaryotic RNAi-like system and methods of use |
摘要 |
Provided herein are methods for inactivating a target polynucleotide. The methods use a psiRNA having a 5' region and a 3' region. The 5' region includes, but is not limited to, 5 to 10 nucleotides chosen from a repeat from a CRISPR locus immediately upstream of a spacer. The 3' region is substantially complementary to a portion of the target polynucleotide. The methods may be practiced in a prokaryotic microbe or in vitro. Also provided are polypeptides that have endonuclease activity in the presence of a psiRNA and a target polynucleotide, and methods for using the polypeptides.
|
申请公布号 |
US8546553(B2) |
申请公布日期 |
2013.10.01 |
申请号 |
US200913055769 |
申请日期 |
2009.07.24 |
申请人 |
TERNS REBECCA;TERNS MICHAEL;HALE CARYN;UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. |
发明人 |
TERNS REBECCA;TERNS MICHAEL;HALE CARYN |
分类号 |
C07H21/04;C12N15/113;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|