发明名称 |
Recombinant hosts and methods for manufacturing polyhydroxyalkanoate |
摘要 |
Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (Ppdc). It was found that the 4HB monomer content in P(3HB-co-4HB) is significantly enhanced to be over 20 mol %, in the range of 8.8 to 23 mol %. |
申请公布号 |
US8546125(B2) |
申请公布日期 |
2013.10.01 |
申请号 |
US201113031350 |
申请日期 |
2011.02.21 |
申请人 |
CHEN GUO-QIANG;LI ZHENG-JUN;SHI ZHEN-YU;GUO YING-YING;JIAN JIA;WU QIONG;TIANJIN GREENBIO MATERIAL CO., LTD. |
发明人 |
CHEN GUO-QIANG;LI ZHENG-JUN;SHI ZHEN-YU;GUO YING-YING;JIAN JIA;WU QIONG |
分类号 |
C12N1/20;C07H21/04;C12P9/00 |
主分类号 |
C12N1/20 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|