发明名称 |
Methods for determination of calcineurin activity and uses in predicting therapeutic outcomes |
摘要 |
The methods of the disclosure provide fluorescence-based assays for calcineurin activity, especially in isolated T cells. The methods include the stimulation of the T cells with agents that specifically target the TCR with or without influencing co-stimulatory pathways. One TCR agonist is monoclonal antibodies specific for CD3, which more precisely distinguish the inducible activity of calcineurin than does an alternative method targeting the T cell receptor (CD3) combined with CD28 costimulation. This method more accurately distinguishes between the measured level of calcineurin activity of T cells from immunosuppressed transplant recipients and normal individuals, and thus has improved diagnostic accuracy with respect to the response of an individual to immunosuppressant therapy following an organ transplant.
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申请公布号 |
US8546095(B2) |
申请公布日期 |
2013.10.01 |
申请号 |
US20100690146 |
申请日期 |
2010.01.20 |
申请人 |
TUMLIN JAMES;KIRK ALLAN D.;ROBERTS BRIAN R.;GOOCH JENNIFER;EMORY UNIVERSITY |
发明人 |
TUMLIN JAMES;KIRK ALLAN D.;ROBERTS BRIAN R.;GOOCH JENNIFER |
分类号 |
G01N33/00;A61K49/00;G01N1/18;G01N33/53 |
主分类号 |
G01N33/00 |
代理机构 |
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代理人 |
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地址 |
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