| 摘要 |
A fluorescence-cued Raman identification system comprises a collection subsystem to collect samples, a reagent treatment subsystem to treat collected samples and a fluorescence imaging subsystem that automatically takes at least one image of the collected sample. The subsystems further include a Raman spectroscopy subsystem that measures the spectrum of viable organisms located from at least one collected image, a visible imaging microscope subsystem that provides a visual image of the particle analyzed by the Raman spectroscopy subsystem and a processor configured to perform image processing to locate viable organisms within the sample, which are targeted by the Raman spectroscopy subsystem. The processor further analyzes the spectrum recorded by the Raman spectroscopy subsystem to make a preliminary identification of the targeted organisms, which can be verified by an operator using the visible imaging microscope subsystem.
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