发明名称 |
USE OF TEMPLATE SWITCHING FOR DNA SYNTHESIS |
摘要 |
<p>A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template/primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non- retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3' end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template/primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.</p> |
申请公布号 |
SG192870(A1) |
申请公布日期 |
2013.09.30 |
申请号 |
SG20130063243 |
申请日期 |
2012.02.23 |
申请人 |
BOARD OF REGENTS |
发明人 |
LAMBOWITZ, ALAN, M.;MOHR, SABINE;WHITE, TRAVIS, B.;KUERSTEN, SCOTT |
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