MODELISATION IN VITRO DES NICHES MEDULLAIRES A CELLULES SOUCHES HEMATOPOIETIQUES : UN OUTIL POUR ETUDIER LA REGULATION DE L'HEMATOPOIESE, EVALUER LE POTENTIEL DE NICHAGE D'UN GREFFON HEMATOPOIETIQUE ET TESTER LA PHARMACO-TOXICOLOGIE DE MEDICAMENTS.

摘要

The present invention relates to a culture support for cultivating hematopoietic stem cells (HSCs) and/or hematopoietic progenitors (HPs), comprising a calcium biomaterial, osteoclasts, endothelial cells and mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes. The present invention also relates to a method for preparing such a culture support, and an in vitro HSC and/or HP cultivation method. The use of such a culture support for studying cellular mechanisms involved in hematopoiesis and/or differentiation of HSC/HPs and/or for studying the efficacy and/or the toxicity of a medicament candidate is also described.