| 摘要 |
A laser microscope includes an objective lens that radiates a laser beam onto a specimen; a stimulation optical system having an LCOS-SLM located at a position optically conjugate with the pupil position of the objective lens and which modulates the phase of the laser beam; and an observation optical system having a galvanometer mirror that scans the laser beam across the specimen, as observation illuminating light, and a PMT that detects the observation light coming from the specimen and collected by the objective lens. A control unit forms a three-dimensional image of the specimen and sets, in that image, stimulation sites in the specimen to be irradiated with a laser beam serving as a stimulation beam by the stimulation optical system at a plurality of different positions in the optical axis direction. The LCOS-SLM modulates the laser beam such that the stimulation sites are irradiated with the laser beam.
|
