ENGINEERING AN INCREASE IN ETHANOL PRODUCTION BY ALTERING COFACTOR SPECIFICITY
摘要
The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.
申请公布号
WO2013141905(A2)
申请公布日期
2013.09.26
申请号
WO2012US67216
申请日期
2012.11.30
申请人
MASCOMA CORPORATION;DARTMOUTH COLLEGE;GUSS, ADAM M.
发明人
GUSS, ADAM M.;LO, JONATHAN;VAN DIJKEN, JOHANNES P.;SHAW, IV, ARTHUR J.;OLSON, DANIEL G.;HERRING, CHRISTOPHER D.