| 摘要 |
A device for performing micro-operations on a vesicular target, comprising an injection pipette (27); a barrel assembly (9); and an outer assembly (1). The injection pipette, barrel assembly, and outer assembly being designed and situated in relation to each other such that an axial vacuum passage is created between the injection pipette and the barrel assembly, and a radial vacuum passage is created between the barrel assembly and outer assembly, and such vacuum passages are isolated from each other and from atmospheric pressure (FIG. 8). The device also comprises a means of advancing and withdrawing the distal end of the barrel assembly in relation to the distal end of the outer assembly so as to create a holding well (31) and a means of advancing the pipette into, and withdrawing the pipette from, a vesicular object. The elements being designed and situated in relationship to each other to allow the simultaneous holding of the vesicular object by applying negative pressure in the radial vacuum passage; injection into or aspiration from the vesicular object via the pipette; and/or aspiration from the vesicular object by applying negative pressure in the axial vacuum passage.
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