ANTHRAPYRIDONE FLUORESCENT DYE N-SUBSTITUTED IN POSITION 4, PREPARATION METHOD AND USE THEREOF

摘要

Disclosed are an anthrapyridone fluorescent dye N-substituted in position 4, the preparation method and use thereof, the compound having a structure as shown in general formula I. In general formula I, n is an integer of 1-10; R is selected from a hydrogen atom, methyl, hydroxyl, amino, dimethylamino, a trimethylammonium halide group and a guanidine hydrochloride group. The anthrapyridone fluorescent dye N-substituted in position 4 in the present invention has a simple synthesis process, good light stability and a relatively long emission wavelength, and has good cell membrane permeability. Thus, another object of the present invention is to provide use of the anthrapyridone fluorescent dye N-substituted in position 4 in the present invention for biological dyeing, such as dyeing in fixed cells, living cells and biological tissues.