Method for the detection of acid production by cariogenic bacteria

摘要

The method comprises optionally concentrating microorganisms contained in a plaque or saliva sample, bringing the microorganisms into contact with a carbon source that is fermented to acid by a cariogenic bacteria, incubating the microorganisms and the carbon source under conditions that facilitate selective acid formation by the cariogenic bacteria, and determining a pH once within a period of 12 hours after addition of the carbon source. The semi-quantitative determination of the cariogenic bacteria in the sample is performed by comparing the determined pH with a reference value. The method comprises optionally concentrating microorganisms contained in a plaque or saliva sample, bringing the microorganisms into contact with a carbon source that is fermented to acid by a cariogenic bacteria, incubating the microorganisms and the carbon source under conditions that facilitate selective acid formation by the cariogenic bacteria, and determining a pH once within a period of 12 hours after addition of the carbon source. The semi-quantitative determination of the cariogenic bacteria in the sample is performed by comparing the determined pH with a reference value. The pH is determined with a pH electrode and is determined using a pH indicator that changes color on acid formation. The incubation step proceeds in the presence of antibiotics that do not significantly impair the growth and/or the acid formation of the cariogenic bacteria, and proceeds in the presence of antibiotics. The step of bringing the microorganisms and/or incubating the microorganisms proceeds in the presence of arginine. Independent claims are included for: (1) a test strip for a semi-quantitative determination of cariogenic bacteria in a plaque or saliva sample; and (2) a test kit for a semi-quantitative determination of cariogenic bacteria in a plaque or saliva sample.