Method for designing siRNA and oligoribonucleotides inhibiting HCV

摘要

The present invention includes methods for designing an siRNA that has efficient RNAi activity against a target gene. The present inventors further focused on siE sequences that have been thought to show RNAi activity against HCV viral RNAs, and mainly selected the D5-50 and D5-197 regions present within the IRES region, and carried on the analysis. As a result, the present inventors successfully identified siRNA sequences that exhibit a more effective RNAi activity against hepatitis C virus RNAs. Furthermore, the siRNAs were demonstrated to have a significant inhibitory effect on HCV propagation in an <Italic>in vivo</Italic> system.