The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to about 20% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
申请公布号
CA2473922(C)
申请公布日期
2013.09.24
申请号
CA20032473922
申请日期
2003.01.22
申请人
WISCONSIN ALUMNI RESEARCH FOUNDATION
发明人
BLATTNER, FREDERICK R.;POSFAI, GYORGY;HERRING, CHRISTOPHER D.;PLUNKETT, GUY D., III;GLASNER, JEREMY D.;TREVOR, TWOSE