LINE OF CELLS CHO[V3D], SECRETING RECOMBINANT VESSEL ENDOTHELIUM GROWTH FACTOR (VEGF) OF HUMANS, ISOFORM A165, WITH EXOGENIC 3×DED-EPITOPE

摘要

FIELD: biotechnologies.SUBSTANCE: by the recombinant method a line of cells CHO[V3D] is produced, which are transformed with plasmid pV3D, coding the human vessel endothelium growth factor, isoform A165, with C-terminal 3xDED-epitope (SEQ ID NO: 1), which is able to produce a biologically active recombinant protein VEGF-A165 of humans with C-terminal 3xDED-epitope in the amount of at least 0.2 mg 1 l of medium for 72 hours with 1 ml of growth medium per 5 cmof single-layer culture. The cell line is deposited in the All-Russian Collection of Industrial Microorganisms VKPM under the registration number VKPM H-123.EFFECT: no impact at biological activity of protein and possibility to do protein treatment.4 dwg, 7 ex