| 摘要 |
FIELD: chemistry.SUBSTANCE: 0.02 ml of collected blood, which is diluted 10 times, are used to prepare 11 test tubes, the first test tube containing 0.2 ml distilled water with 2.5 mM CaCl(control); the next five test tubes contain 0.2 ml distilled water which contains, besides 2.5 mM CaCl, adrenalin in concentration corresponding to each test tube (10, 10, 10, 10, 10g/ml); and the remaining five test tubes contain, besides 0.2 ml distilled water and 2.5 mM CaCl, 10g/ml adrenergic blockers (atenolol and nicergoline) and adrenalin in concentration corresponding to each test tube (10, 10, 10, 10, 10g/ml). Further, 0.02 ml of blood are added to each test tube, and after 45 s, 0.2 ml of 6% NaCl is added in ratio of 1:1. The number of non-hemolysed erythrocytes in each test tube is counted. Increase in non-hemolysed erythrocytes indicates domination of ?-adrenoreactivity and a decrease indicates domination of ?-adrenoreactivity.EFFECT: high accuracy of estimation.1 tbl, 7 dwg |
