COMPOSITIONS AND METHODS FOR ANALYZING HISTIDINE PHOSPHORYLATION
摘要
A peptide of the general structure: Z-W-Y, wherein Z and Y are independently a one to eight amino acid sequence wherein the amino acids are selected from glycine and alanine and W is a non-hydrolyzable pHis analogue. Such peptides can be used to produce sequence-independent anti-phosphohistidine antibodies. Also provided is a composition comprising antibodies that specifically bind to a peptide comprising a phosphohistidine (or a non-hydrolyzable pHis analogue) but fail to specifically bind to an identical peptide containing histidine instead of phosphohistidine.
申请公布号
WO2013138474(A1)
申请公布日期
2013.09.19
申请号
WO2013US30917
申请日期
2013.03.13
申请人
THE SALK INSTITUTE FOR BIOLOGICAL STUDIES;SANOFI;STANKOVA, MAGDA;AL-OBEIDI, FAHAD;MAUGER, JACQUES;BINNIE, ROBERT, A