CELL SHEET, CELL CULTURE METHOD, AND CELL CULTURE APPARATUS

摘要

A cell culture method characterized by culturing a stem cell or a progenitor cell that can form a tissue for a first period, which is a portion of a period for causing the self-replication of the stem cell or the progenitor cell and included in a period for culturing of the stem cell or the progenitor cell, while supplying oxygen in a first supply amount, then changing the amount of oxygen to be supplied from the first supply amount to a second supply amount which is higher than the first supply amount on the basis of the degree of the proliferation of the stem cell or the progenitor cell by self-replication, and then culturing the stem cell or the progenitor cell for a second period, which is a period including a period for causing the differentiation of the stem cell, while supplying oxygen in the above-mentioned second supply amount, wherein no feeder cell is used during the first period and the second period.