YEAST EXPRESSION VECTOR FOR YEAST CHROMOSOMAL INTEGRATION OR TRANSFORMATION OF INDUSTRIAL STRAINS, AND USE THEREOF

摘要

The present invention relates to a novel dual module expression vector for yeast transformation, and a transformation method of a target nucleotide sequence using the same. An expression module of the present invention comprises: an expression module comprising a multiple cloning site (MCS), a proper promoter, and a terminator; and a rescue module. The vector of the present invention can stably express a target gene in a yeast, particularly, Kluyveromyces marxianus or Saccharomyces cerevisiae, and can integrate a target gene to a specific position in a chromosome with high efficiency. A transformation method using the vector of the present invention can be very usefully applied to industrial production of desired products (biomass) since it is possible to prepare a transgenic yeast strain in which a target gene is stably integrated in a chromosome, and then to carry out continuous transformation for inducing the modification of another target gene to the yeast strain.