发明名称 |
5-FLUOROURACIL-RESISTANT BACTERIA AND METHOD FOR PRODUCTION THEREOF |
摘要 |
<p>The present invention provides a method for producing a cytosine deaminase (CD)-expressing, 5-fluorouracil (5-FU)-resistant microorganism which can grow in anaerobic tumor tissues, can express CD, and has a resistance to 5-FU at a concentration that is at least effective for antitumor activity. More specifically, the method is a method (A) comprising performing subculture or acclimation culture of a CD-expressing microorganism which can grow in anaerobic tumor tissues, in the presence of 5-fluorocytosine (5-FC), or a method (B) comprising (1) performing subculture or acclimation culture of a microorganism which can grow in anaerobic tumor tissues and does not express CD, in the presence of 5-FU to produce a 5-FU-resistant microorganism and (2) transforming the 5-FU-resistant microorganism by introducing a CD gene. The present invention also provides the CD-expressing, 5-FU-resistant microorganism and a pharmaceutical composition comprising the microorganism.</p> |
申请公布号 |
EP1867714(B1) |
申请公布日期 |
2013.09.18 |
申请号 |
EP20060731050 |
申请日期 |
2006.04.04 |
申请人 |
ANAEROPHARMA SCIENCE INC. |
发明人 |
TANIGUCHI, SHUNICHIROU;AMANO, JUN;FUJIMORI, MINORU;HAMAJI, YOSHINORI |
分类号 |
C12N1/21;C12N5/10;C12N15/09 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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