| 摘要 |
A monostatic fluorometer comprises light source 30 to transmit an excitation beam 110; detector 60 to detect fluorescent emissions 130; and beamsplitter 90 to direct the excitation beam over an excitation path 120 into a fluorescent target 100 to generate emissions; the beamsplitter receiving the emissions over an emission path defined by a numerical aperture of the detector 60 and the beamsplitter 90 which is parallel to the excitation path, the emission beam passing through the beamsplitter and onto the detector. The beamsplitter enables the excitation beam to be coaxially aligned with an emission beam received by a detector which increases overlap volume of the device and enables measurements at distances closer to the device than possible with a multistatic device which increases sensitivity. The fluorometer can be made more compact than is possible with a multistatic device and can be air-free which makes it suitable for submersible applications.
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