| 摘要 |
Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42). |
