发明名称 |
METHOD FOR PRODUCING INTESTINAL CELLS |
摘要 |
An object of the present invention is to provide a method of producing intestinal cells by use of pluripotent stem cells as a starting material. According to the present invention, provided is a method of producing intestinal cells, comprising the steps of: (A) inducing differentiation of pluripotent stem cells into definitive endoderm cells; and (B) culturing the definitive endoderm cells in the presence of (2'Z, 3'E)-6-bromoindirubin-3-oxime (BIO) and N-[(3,5-difluorophenyl)acetyl]-L-Ala-2-phenyl-L-Gly-tert-butyl-OH (DAPT) to thereby induce differentiation of the definitive endoderm cells into intestinal cells. |
申请公布号 |
KR20130101557(A) |
申请公布日期 |
2013.09.13 |
申请号 |
KR20137013739 |
申请日期 |
2011.10.31 |
申请人 |
NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY;LSIP, LLC |
发明人 |
KUME SHOEN;OGAKI SOICHIRO;SHIRAKI NOBUAKI;KUME KAZUHIKO |
分类号 |
C12N5/071;C12N5/02;C12Q1/04;G01N33/15 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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