ASSAY FOR THE DETECTION OF NUCLEOTIDE SUBSTITUTIONS IN GENOMES OF SABIN ORAL POLIOVIRUS VACCINE VIRUSES

摘要

The present invention provides a multiplex SNP assay method and primers for the specific and simultaneous detection of mutations in the genomes of live attenuated oral poliovirus vaccine (Sabin) viruses, vaccine-like and vaccine derived polio viruses of all the three serotypes. Importantly, the inventive primers and the assay eliminate the need for costly, time-consuming and complex procedures like complete genome sequencing. This novel assay is able to test several samples in a very short time. Therefore, the assay is very simple, fast and cost-effective. A kit comprising the novel primers is also provided.