发明名称 |
IMPROVED METHODS OF NUCLEIC ACID SEQUENCING |
摘要 |
Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand. |
申请公布号 |
WO2013131962(A1) |
申请公布日期 |
2013.09.12 |
申请号 |
WO2013EP54517 |
申请日期 |
2013.03.06 |
申请人 |
ILLUMINA CAMBRIDGE LIMITED |
发明人 |
GORMLEY, NIALL ANTHONY;FRASER, LOUISE;KOKKO-GONZALES, PAULA |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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