| 摘要 |
Method (M1) for finding a sample having a disordered von Willebrand factor-platelet glycoprotein Ib (vWF-GPIb) interaction, involves (a) contacting the sample with isolated GPIbalpha protein, vWF protein and solid phase associated with antibody specific for the isolated GPIbalpha protein, and (b) determining formation of a complex between vWF protein, GPIbalpha protein and the solid phase. Independent claims are included for the following: (1) test kit for carrying out the above-mentioned method (M1), involves first reagent containing isolated GPIbalpha protein, a second reagent containing vWF protein, and a third reagent containing a solid phase, preferably a particulate solid phase, which is associated with an antibody specific for the isolated GPIbalpha protein; and (2) method (M2) for determining the concentration of glycocalicin in sample, involves (a) contacting the sample with isolated GPIbalpha protein, vWF protein and solid phase associated with an antibody specific for the isolated GPIbalpha protein, and (b) determining formation of a complex between vWF protein, GPIbalpha protein and the solid phase, where the sample is contacted with vWF protein, and hence final concentration of at least 50% of the norm VWF activity, preferably more than 100% of the norm VWF activity, is obtained in the assay mix in which complex formation is determined. |
